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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
17/08/2023 |
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Actualizado : |
17/08/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FERNÁNDEZ, M.; CASAUX, M.L.; FRAGA, M.; VIGNOLI, R.; BADO, I.; ZUNINO, P.; UMPIÉRREZ, A. |
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Afiliación : |
MAGALÍ FERNÁNDEZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Facultad de Medicina, UdelaR, Av Alfredo Navarro 3051, Montevideo 11600, Uruguay; INÉS BADO, Departamento de Bacteriología y Virología, Facultad de Medicina, UdelaR, Av Alfredo Navarro 3051, Montevideo 11600, Uruguay; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay; ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay. |
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Título : |
Shiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Microorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS. |
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ISSN : |
2076-2607 (electronic). |
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DOI : |
10.3390/microorganisms11071704 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford,
Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A. McAllister and Gillian Tarr. -- This article belongs to the Special Issue The Science of Shiga Toxin-Producing (Verotoxin-Producing) Escherichia coli (STEC): An Ongoing One Health Journey toward Improved Health and Food Safety) (https://www.mdpi.com/journal/microorganisms/special_issues/STEC_VTEC ) -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- FUNDING: This research was partially funded by the project PL-15 from "Instituto Nacional de Investigación Agropecuaria" (INIA).. -- Supplementary imaterials an be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms11071704/s1. Table S1: Origin of the animals. Table S2: Virulence profiles in E. coli isolates recovered from dead animals (n = 221). |
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Contenido : |
ABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalence of multi-resistance among STEC and qnrB genes was determined. The PFGE showed a high diversity of pathogenic strains with similar genetic profiles. It can be speculated that EHEC (stx1/eae/ehxA) could play an important role in mortality. The afa8E, f17G1 and papC genes could also have a role in calf mortality. Multidrug resistance defies disease treatment and increases the risk of death, while the potential transmissibility of genes to other species constitutes a threat to public health. © 2023 by the authors. Licensee MDPI, Basel, Switzerland. MenosABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalen... Presentar Todo |
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Palabras claves : |
Dairy calf; MDR STEC; Mortality; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Shiga toxin-producing E. coli. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
https://www.mdpi.com/2076-2607/11/7/1704/pdf
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Marc : |
LEADER 04099naa a2200289 a 4500
001 1064289
005 2023-08-17
008 2023 bl uuuu u00u1 u #d
022 $a2076-2607 (electronic).
024 7 $a10.3390/microorganisms11071704$2DOI
100 1 $aFERNÁNDEZ, M.
245 $aShiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford, Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A. McAllister and Gillian Tarr. -- This article belongs to the Special Issue The Science of Shiga Toxin-Producing (Verotoxin-Producing) Escherichia coli (STEC): An Ongoing One Health Journey toward Improved Health and Food Safety) (https://www.mdpi.com/journal/microorganisms/special_issues/STEC_VTEC ) -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- FUNDING: This research was partially funded by the project PL-15 from "Instituto Nacional de Investigación Agropecuaria" (INIA).. -- Supplementary imaterials an be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms11071704/s1. Table S1: Origin of the animals. Table S2: Virulence profiles in E. coli isolates recovered from dead animals (n = 221).
520 $aABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalence of multi-resistance among STEC and qnrB genes was determined. The PFGE showed a high diversity of pathogenic strains with similar genetic profiles. It can be speculated that EHEC (stx1/eae/ehxA) could play an important role in mortality. The afa8E, f17G1 and papC genes could also have a role in calf mortality. Multidrug resistance defies disease treatment and increases the risk of death, while the potential transmissibility of genes to other species constitutes a threat to public health. © 2023 by the authors. Licensee MDPI, Basel, Switzerland.
653 $aDairy calf
653 $aMDR STEC
653 $aMortality
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aShiga toxin-producing E. coli
700 1 $aCASAUX, M.L.
700 1 $aFRAGA, M.
700 1 $aVIGNOLI, R.
700 1 $aBADO, I.
700 1 $aZUNINO, P.
700 1 $aUMPIÉRREZ, A.
773 $tMicroorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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| Registros recuperados : 37 | |
| 21. |  | PARUELO, J.M.; PIÑEIRO, G.; BALDI, G.; BAEZA, S.; LEZAMA, F.; ALTESOR, A.; OESTERHELD, M. Carbon stocks and fluxes in rangelands of the Río de la plata basin. Rangeland Ecology & Management, 2010, v. 63, no. 1 p. 94-108. 63 Article history: Manuscript received 17 March 2008; manuscript accepted 3 April 2009.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 2 |
| Biblioteca(s): INIA Treinta y Tres. |
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| 23. | | JAURENA, M.; DE BARBIERI, I.; LEZAMA, F.; AYALA, W.; MONTOSSI, F.; RIVAS, M. Atributos de pasturas naturales sobre suelos superficiales de Basalto con cargas contrastantes de capones ln: Reunión del grupo técnico en forrajeras del Cono Sur, 22., 2008, Minas, Uruguay Bioma campos: innovando para mantener su sustentabilidad y competitividad. Memorias. Minas (Uruguay): INIA; FAO; PROCISUR, 2008. p. 199 Versión impresa y en CD ROM Instituto Nacional de Investigación Agropecuaria, Uruguay; FAO; PROCISUR| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó; INIA Treinta y Tres. |
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| 24. | | JAURENA, M.; LEZAMA, F.; SALVO, L.; CARDOZO, G.; AYALA, W.; TERRA, J.A.; NABINGER, C. The dilemma of improving native grasslands by overseeding legumes: production intensification or diversity conservation. Rangeland Ecology & Management, 2016, v. 69, no. 1 p. 35-42. Article history: Received 25 November 2014; Accepted 23 September 2015.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Tacuarembó; INIA Treinta y Tres. |
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| 25. | | JAURENA, M.; SALVO, L.; TERRA, J.A.; AYALA, W.; BARRIOS, E.; LEZAMA, F. Cambios en el largo plazo de características de suelo y vegetación de mejoramientos de campo natural. ln: Reunión del Grupo Técnico en Forrajeras del Cono Sur, Grupo Campos, 22, 2008, Minas, Uruguay Ayala, W.; Lezama, F.; Barrios, E.; Bemhaja, M.; Saravia, H.; Formoso, D.; Boggiano, P., ed. Bioma campos : Innovando para mantener su sustentabilidad y competitividad. Memorias. Minas (Uruguay): Grupo Campos, 2008.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Treinta y Tres. |
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| 27. | | Jaurena, M.; Salvo, L.; Terra, J.; Ayala, W.; Bermúdez, R.; Barrios, E.; Lezama, F. Microcuencas apareadas en Tacuarembó: contribución de raíces de comunidades de campo natural pastoreado y sombreado con Pinus taeda ln: Reunión del grupo técnico en forrajeras del Cono Sur, 22., 2008, Minas, Uruguay Bioma campos: innovando para mantener su sustentabilidad y competitividad. Memorias. Minas (Uruguay): INIA; FAO; PROCISUR, 2008. p. 188 Versión impresa y en CD ROM Instituto Nacional de Investigación Agropecuaria, Uruguay; FAO; PROCISUR| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 28. | | CARDOZO, G.; AYALA, W.; LEZAMA, F.; REYNO, R.; CUADRO, R.; MICHELINI, D.; JAURENA, M.; LATTANZI, F. Limited response to phosphorus fertilization in native South American grasslands across a productivity gradient despite low phosphorus concentrations in grassland herbage. Plant and Soil. 2024. https://doi.org/10.1007/s11104-024-06562-5 Article history: Received 10 October 2023; Accepted 14 February 2024; Published 26 February 2024. -- Responsible Editor: N. Jim Barrow. --
Correspondence: G.A. Cardozo, Instituto Nacional de Investigación Agropecuaria (INIA), Estación...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 31. | | TOMMASINO, A.; LEZAMA, F.; GALLEGO, F.; CAMBA SANS, G.; PARUELO, J. Rangeland resilience to droughts: Changes across an intensification gradient. Applied Vegetation Science. Special Issue: Grazzing and Vegetation. 2023, Vol.26, Issue2, e12722. https://doi.org/10.1111/avsc.12722 Article history: Received 28 October 2022, Revised 3 March 2023, Accepted 7 March 2023, First published 17 March 2023. -- Correspondence author: Andrea Tommasino, Instituto de Ecología y Ciencias Ambientales, Facultad de Ciencias,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 32. | | PARUELO, J.; AYALA, W.; TOMMASINO, A.; MESA, D.; DOMINGUEZ, M.; BAS, R.; LEZAMA, F. El pastoreo es más que comer pasto. La simulación del pisoteo del ganado. Pasturas. Revista INIA Uruguay, Diciembre 2021, no.67, p.38-40. (Revista INIA; 67).| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas. |
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| 33. | | JAURENA, M.; OLMOS, F.; BEMHAJA, M.; BERRETTA, E.J.; LEZAMA, F.; PEREZ GOMAR, E.; ZERBINO, M.; CONDON, F.; BLUMETTO, O.; CASTAÑO, J.; ROCANOVA, M. Campo natural en Uruguay: biodiversidad y manejo para la sustentabilidad. Suplemento Tecnológico INIA, 2008, p. 42-43. Suplemento tecnológico Expo Prado 2008.| Biblioteca(s): INIA Treinta y Tres. |
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| 34. | | JAURENA, M.; OLMOS, F.; BEMHAJA, M.; BERRETTA, E.; LEZAMA, F.; PEREZ GOMAR, E.; ZERBINO, M.; CONDON, F.; BLUMETTO, O.; CASTAÑO, J.; ROCANOVA, M. Campo natural en Uruguay: biodiversidad y manejo para la sustentabilidad. Suplemento Tecnológico INIA, 2008, p. 42-43. Suplemento tecnológico Expo Prado 2008.| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Tacuarembó. |
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| 35. | | ALTESOR, A.; GALLEGO, F.; FERRÓN, M; PEZZANI, F.; LÓPEZ-MÁRSICO, L.; LEZAMA,F.; BAEZA, S.; PEREIRA, M.; COSTA, B.; PARUELO, J. An inductive approach to build State-and-Transition Models for Uruguayan grasslands. Rangeland Ecology and Management, November 2019, Volume 72, Issue 6, Pages 1005-1016. Doi: https://doi.org/10.1016/j.rama.2019.06.004 Article history: Received 19 October 2018// Revised 7 June 2019// Accepted 24 June 2019// Available online 8 August 2019.-Correspondence: E-mail address: aaltesor@fcien.edu.uy (A. Altesor).This work was supported by Instituto Nacional de...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 36. | | JAURENA, M.; DE BARBIERI, I.; MONTOSSI, F.; LEZAMA, F.; PEREZ GOMAR, E.; DIAZ, S.; SOSA, M.; CACERES, J.; ROVIRA, F.; ALBERNAZ, F.; SANCRISTOBAL, E.; LIMA, G. Evaluación de diferentes ofertas de forraje de campo natural sobre la producción y calidad de lanas finas y superfinas y en indicadores de sustentabilidad de suelos y de la comulnidad vegetal. Día de campo, INIA Tacuarembó, Unidad Experimental Glencoe, Setiembre, 2008. Alternativas de intensificación, especialización, diversificación y valorización de la ganadería ovina y bovina en basalto. Tacuarembó (Uruguay): INIA, 2008. p. 8-10| Biblioteca(s): INIA Tacuarembó. |
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| 37. | | REUNIÓN DEL GRUPO TÉCNICO EN FORRAJERAS DEL CONO SUR, GRUPO CAMPOS, 22, 2008, MINAS, URUGUAY AYALA, W.; LEZAMA, F.; BARRIOS, E.; BEMHAJA, M.; SARAVIA, H.; FORMOSO, D.; BOGGIANO, P., ED. Bioma Campos: innovando para mantener su sustentabilidad y competitividad. Memorias Minas (Uruguay): Grupo Campos, 2008. 276 p Versión impresa y en CD ROM| Tipo: Ediciones Especiales |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas. |
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| Registros recuperados : 37 | |
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